FGFR2 Protein, Human, Recombinant (alpha IIIb, hFc)

FGFR2 Protein, Human, Recombinant (alpha IIIb, hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 66.3 kDa and the accession number is P21802-17.

FGFR2 Protein, Human, Recombinant (alpha IIIb, hFc)

パッケージサイズ	在庫状況	単価(税別)
200 μg	在庫あり	￥ 134,000

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生物学的情報	1. Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.5-4 ng/mL. 2. Measured by its ability to bind recombinant Fc-FGF10 in a functional ELISA.
説明	FGFR2 Protein, Human, Recombinant (alpha IIIb, hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 66.3 kDa and the accession number is P21802-17.

Species	Human
Expression Host	HEK293
Tag	hFc
Accession Number	P21802-17
別名	BFR-1, KGFR, TK25, JWS, ECT1, K-SAM, FGFR2 α(IIIb), CD332, fibroblast growth factor receptor 2, BEK, CFD1, TK14, BBDS, CEK3
Construction	A DNA sequence encoding the human FGFR2(alpha(IIIb)) (NP_001138385.1) (Met1-Glu378) was expressed with the Fc region of human IgG1 at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	66.3 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Marie PJ,et al.(2003) Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 and BMP-2 signaling. Histol. 17(3):877-85. 2. Park WJ,et al.(1996) Novel FGFR2 mutations in Crouzon and Jackson-Weiss syndromes show allelic heterogeneity and phenotypic variability. Hum Mol Genet. 4(7):1229-33. 3. Orr-Urtreger A,et al.(1993) Developmental localization of the splicing alternatives of fibroblast growth factor receptor-2 (FGFR2). Dev Biol. 158(2):475-86.

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FGFR2 Protein, Human, Recombinant (alpha IIIb, hFc)

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