FGFR2 Protein, Human, Recombinant (aa 1-378, His)

FGFR2 Protein, Human, Recombinant (aa 1-378, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 41.1 kDa and the accession number is P21802-3.

FGFR2 Protein, Human, Recombinant (aa 1-378, His)

パッケージサイズ	在庫状況	単価(税別)
200 μg	約5 days	￥ 207,500

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説明	FGFR2 Protein, Human, Recombinant (aa 1-378, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 41.1 kDa and the accession number is P21802-3.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P21802-3
別名	TK25, ECT1, JWS, fibroblast growth factor receptor 2, CD332, CEK3, K-SAM, BFR-1, BBDS, KGFR, CFD1, TK14, BEK
Construction	The Human FGFR2-alpha(IIIb) (NP_075259.4) (Met1-Glu378) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE.≥90 % as determined by SEC-HPLC.
分子量	41.1 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Marie PJ,et al.(2003) Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 and BMP-2 signaling. Histol. 17(3):877-85. 2. Park WJ,et al.(1996) Novel FGFR2 mutations in Crouzon and Jackson-Weiss syndromes show allelic heterogeneity and phenotypic variability. Hum Mol Genet. 4(7):1229-33. 3. Orr-Urtreger A,et al.(1993) Developmental localization of the splicing alternatives of fibroblast growth factor receptor-2 (FGFR2). Dev Biol. 158(2):475-86.

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FGFR2 Protein, Human, Recombinant (aa 1-378, His)

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