FLT3 Ligand Protein, Human, Recombinant (His)

FLT3 Ligand Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 20.2 kDa and the accession number is P49771-1.

FLT3 Ligand Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
10 μg	在庫あり	￥ 24,500
20 μg	約5 days	￥ 32,000
50 μg	約5 days	￥ 45,500
100 μg	約5 days	￥ 79,500
200 μg	約5 days	￥ 141,000
500 μg	約5 days	￥ 298,500

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生物学的情報	Measured in a cell proliferation assay using BaF3 mouse proB cells transfected with mouse Flt3. The ED50 for this effect is typically 2-11 ng/mL.
説明	FLT3 Ligand Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 20.2 kDa and the accession number is P49771-1.

Species	Human
Expression Host	Baculovirus-Insect Cells
Tag	His
Accession Number	P49771-1
別名	fms related tyrosine kinase 3 ligand, FLT3L, FLT3LG, FL
Construction	A DNA sequence encoding the human FLT3LG (P49771-1) (Thr27-Pro185) was expressed, with a polyhistidine tag at the N-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	20.2 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile 20mM Tris, 300mM NaCl,10% glycerol, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 -positive classical DCs and their CD103 -positive tissue counterparts.

参考文献

1. Hannum C, et al. (1994) Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs. Nature 368 (6472): 643-8. 2. Lyman SD, et al. (1995) Identification of soluble and membrane-bound isoforms of the murine flt3 ligand generated by alternative splicing of mRNAs. Oncogene 10 (1): 149-57. 3. Lyman SD, et al. (1994) Molecular cloning of a ligand for the flt3/flk-2 tyrosine kinase receptor: a proliferative factor for primitive hematopoietic cells. Cell 75 (6): 1157-67.

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FLT3 Ligand Protein, Human, Recombinant (His)

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