FOLR1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 55-75 kDa and the accession number is P15328.
| 生物学的情報 |
Loaded Human FOLR1-Fc on APS Biosensor, can bind Anti-Human FOLR1 mAb with an affinity constant of 0.11 nM as determined in BLI assay. (Regularly tested)
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| 説明 |
FOLR1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 55-75 kDa and the accession number is P15328. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-hFc
|
| Accession Number |
P15328
|
| 別名 |
Folate receptor 1, Folate receptor, Folate receptor alpha, FOLR1, FR-alpha, Adult folate-binding protein, FR-α, Folate receptor α, Ovarian tumor-associated antigen MOv18, FBP
|
| Amino Acid |
Arg25-Ser234
|
| Construction |
Arg25-Ser234
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
|
| 分子量 |
55-75 kDa, reducing conditions
|
| Endotoxin |
< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
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| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
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| Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
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| Research Background |
Folate receptor alpha(FOLR) belongs to the folate receptor family, and is primarily expressed in tissues of epithelial origin. It is also expressed in kidney, lung and cerebellum. The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. It has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. It is required for normal embryonic development and normal cell proliferation.
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