Frizzled 5 Protein, Human, Recombinant (His)

Frizzled 5 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.6 kDa and the accession number is Q13467.

Frizzled 5 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 103,500
200 μg	約5 days	￥ 183,500
500 μg	約5 days	￥ 388,000

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生物学的情報	Immobilized Recombinant Human Frizzled-5 / FZD5 Protein (His Tag) at 2μg/mL (100μL/well) can bind pan-FZD antibody, Human IgG2 , the EC50 is 12-36 ng/mL.
説明	Frizzled 5 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 17.6 kDa and the accession number is Q13467.

Species	Human
Expression Host	HEK293
Tag	His
Accession Number	Q13467
別名	C2orf31, frizzled class receptor 5, HFZ5
Construction	A DNA sequence encoding the extracellular domain (Met 1-Pro 167) of human frizzled 5 (NP_003459.2) was fused with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	17.6 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 1 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.Frizzled 5 (FZD5) is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt1B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus.

参考文献

1. Wang Y.,et al.,(1996), A large family of putative transmembrane receptors homologous to the product of the Drosophila tissue polarity gene frizzled. J. Biol. Chem. 271:4468-4476. 2. Saitoh T.,et al., (2001), Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region.Int. J. Oncol. 19:105-110. 3. Ota T.,et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.

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Frizzled 5 Protein, Human, Recombinant (His)

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