Frizzled-8 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 22-32 KDa and the accession number is Q9H461.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
10 μg | 約5 days | ¥ 33,000 |
50 μg | 約5 days | ¥ 100,000 |
500 μg | 約5 days | ¥ 562,500 |
1 mg | 約5 days | ¥ 844,500 |
説明 | Frizzled-8 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 22-32 KDa and the accession number is Q9H461. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-6xHis |
Accession Number | Q9H461 |
別名 | FZD8, hFZ8, frizzled family receptor 8, frizzled 8, FZ-8, Frizzled8, Frizzled-8 |
Amino Acid | Ala28-Pro172 |
Construction | Ala28-Pro172 |
Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 22-32 KDa, reducing conditions |
Endotoxin | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas. |
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Please read the User Guide of Recombinant Proteins for more specific information.
Frizzled-8 Protein, Human, Recombinant (His) FZD8 FZD 8 hFZ8 frizzled family receptor 8 FZ 8 frizzled 8 FZ-8 Frizzled8 FZ8 Frizzled-8 FZD-8 recombinant recombinant-proteins proteins protein