FSHB Protein, Mouse, Recombinant (hFc)

Fertility is dependent on follicle-stimulating hormone (FSH), a product of gonadotrope cells of the anterior pituitary gland. Hypothalamic gonadotropin-releasing hormone (GnRH) and intra-pituitary activins are regarded as the primary drivers of FSH synthesis and secretion. Both stimulate expression of the FSH beta subunit gene (Fshb), although the underlying mechanisms of GnRH action are poorly described relative to those of the activins. FSHB Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 39.2 kDa and the accession number is Q60687.

FSHB Protein, Mouse, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 96,000
500 μg	約5 days	￥ 383,500
1 mg	約5 days	￥ 643,000

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説明

Species	Mouse
Expression Host	HEK293 Cells
Tag	C-hFc
Accession Number	Q60687
別名	FSH-beta, FSH-B, FSHB, FSH-β, Follitropin
Construction	His20-Glu130
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	39.2 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Fertility is dependent on follicle-stimulating hormone (FSH), a product of gonadotrope cells of the anterior pituitary gland. Hypothalamic gonadotropin-releasing hormone (GnRH) and intra-pituitary activins are regarded as the primary drivers of FSH synthesis and secretion. Both stimulate expression of the FSH beta subunit gene (Fshb), although the underlying mechanisms of GnRH action are poorly described relative to those of the activins.

参考文献

1. Schang G, et al. HDAC inhibitors impair Fshb subunit expression in murine gonadotrope cells. J Mol Endocrinol. 2019 Feb 1;62(2):67-78. doi: 10.1530/JME-18-0145. PMID: 30481159.

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FSHB Protein, Mouse, Recombinant (hFc)

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