GAPDH Protein, Human, Recombinant (His)

GAPDH Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 38 kDa and the accession number is P04406.

GAPDH Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

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説明	GAPDH Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 38 kDa and the accession number is P04406.

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	P04406
別名	G3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase
Construction	The Human GAPDH (P04406) (Met 1-Glu 335) was expressed, with a polyhistidine tag at the N-terminus.
Protein Purity	> 93 % as determined by SDS-PAGE
分子量	38 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 50 mM Tris, 30% glycerol, pH 7.5.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

参考文献

1. Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9. 2. HAra MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9. 3. Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20. 4. Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.

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GAPDH Protein, Human, Recombinant (His)

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