GDF-15 Protein, Human, Recombinant (hFc)

GDF-15 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 40.6 kDa and the accession number is Q99988.

GDF-15 Protein, Human, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 45,500
100 μg	約5 days	￥ 77,000
200 μg	約5 days	￥ 131,500
500 μg	約5 days	￥ 415,500

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生物学的情報	Testing in progress
説明	GDF-15 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 40.6 kDa and the accession number is Q99988.

Species	Human
Expression Host	HEK293 Cells
Tag	N-hFc
Accession Number	Q99988
別名	PTGFB, PLAB, MIC-1, MIC1, PDF, NAG-1, growth differentiation factor 15, GDF-15
Construction	The mature form of Human GDF15 (NP_004855.2) (Ala 197-Ile 308) was expressed with the fused Fc region of Human IgG1 at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	40.6 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Growth-differentiation factor 15 (GDF15), also known as MIC-1, is a secreted member of the transforming growth factor (TGF)-β superfamily, as a novel antihypertrophic regulatory factor in the heart. GDF-15 / GDF15 is not expressed in the normal adult heart but is induced in response to conditions that promote hypertrophy and dilated cardiomyopathy and it is expressed highly in liver. GDF-15 / GDF15 has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF-15 / GDF15 is synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein. GDF-15 / GDF15 overexpression arising from an expanded erythroid compartment contributes to iron overload in thalassemia syndromes by inhibiting hepcidin expression.

参考文献

1. Ago T, et al. (2006) GDF15, a cardioprotective TGF-beta superfamily protein. Circ Res. 98 (3): 294-297. 2. Hsiao E, et al. (2000) Characterization of growth-differentiation factor 15, a transforming growth factor beta superfamily member induced following liver injury. Mol Cell Biol. 20 (10): 3742-51. 3. Zimmers T, et al. (2005) Growth differentiation factor-15/macrophage inhibitory cytokine-1 induction after kidney and lung injury. Shock. 23 (6): 543-8.

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GDF-15 Protein, Human, Recombinant (hFc)

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