GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His)

GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 28.7 kDa and the accession number is AAB65663.

GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 58,500
200 μg	約5 days	￥ 100,000

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生物学的情報	Activity Assay
説明	GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 28.7 kDa and the accession number is AAB65663.

Species	Aequorea victoria
Expression Host	E. coli
Tag	His
Accession Number	AAB65663
別名	GFP Protein
Construction	A DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser2-Lys238) was expressed with a polyhistide tag at the N-terminus.
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
分子量	28.7 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile PBS, pH7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.

参考文献

1. Evdokimovet al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637. 2. HAaset al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248. 3. Kremerset al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067. 4. Liet al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028. 5. Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161. 6. Shaginet al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.

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GFP Protein, Aequorea victoria, Recombinant (aa 2-238, His)

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