GFPT1 Protein, Human, Recombinant

カタログ番号 TMPY-01189

別名: GFAT1, CMSTA1, GFAT, glutamine--fructose-6-phosphate transaminase 1, GFPT1L, MSLG, GFA, GFAT1m, GFPT

Glutamine:fructose-6-phosphate amidotransferase 1 (GFAT), also known as GFPT1, is a member of the N-terminal nucleophile aminotransferases and the first rate-limiting enzyme for the entry of glucose into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT transfers the amino group from the L-glutamine amide to the D-fructose 6-phosphate, producing glutamic acid and glucosamine 6-phosphate. GFAT exists as a homotetramer in cytoplasm, and is proposed to be most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. The full length of human GFAT contains 1 glutamine amidotransferase type-2 domain which catalyzes amide nitrogen transfer from glutamine to the appropriate substrate, and 2 SIS (Sugar Isomerase) domains found in many phosphosugar isomerases and phosphosugar binding proteins.Two isoforms of gfat have been identified: GFAT1 is predominantly expressed in skeletal muscle, whereas GFAT2 is expressed mainly in the central nervous system.

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GFPT1 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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Product Properties

参考文献

説明

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	AAA58502.1
別名	GFAT1, CMSTA1, GFAT, glutamine--fructose-6-phosphate transaminase 1, GFPT1L, MSLG, GFA, GFAT1m, GFPT
Construction	The sequence corresponding to amino acids (Gln 332-Glu 699) of Human GFAT (AAA58502.1) was expressed and purified with two amino acids (Gly & Pro) at the N-terminus.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	41.5 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Glutamine:fructose-6-phosphate amidotransferase 1 (GFAT), also known as GFPT1, is a member of the N-terminal nucleophile aminotransferases and the first rate-limiting enzyme for the entry of glucose into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT transfers the amino group from the L-glutamine amide to the D-fructose 6-phosphate, producing glutamic acid and glucosamine 6-phosphate. GFAT exists as a homotetramer in cytoplasm, and is proposed to be most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. The full length of human GFAT contains 1 glutamine amidotransferase type-2 domain which catalyzes amide nitrogen transfer from glutamine to the appropriate substrate, and 2 SIS (Sugar Isomerase) domains found in many phosphosugar isomerases and phosphosugar binding proteins.Two isoforms of gfat have been identified: GFAT1 is predominantly expressed in skeletal muscle, whereas GFAT2 is expressed mainly in the central nervous system.

参考文献

1. McKnight G.L.,et al.,(1992), Molecular cloning, cDNA sequence, and bacterial expression of human glutamine:fructose-6-phosphate amidotransferase. J. Biol. Chem. 267:25208-25212. 2. Hillier L.W.,et al., (2005), Generation and annotation of the DNA sequences of human chromosomes 2 and 4.Nature 434:724-731. 3. Beausoleil S.A.,et al.,(2006), A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat. Biotechnol. 24:1285-1292.

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Keywords

GFPT1 Protein, Human, Recombinant GFAT 1 GFAT1 CMSTA1 GFAT glutamine--fructose-6-phosphate transaminase 1 GFPT1L MSLG GFAT-1 GFA GFAT1m CMSTA 1 CMSTA-1 GFPT recombinant recombinant-proteins proteins protein