GFR Alpha-3/GFRA3 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 67.3 kDa and the accession number is O60609-1.
説明 | GFR Alpha-3/GFRA3 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 67.3 kDa and the accession number is O60609-1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | His, hFc |
Accession Number | O60609-1 |
別名 | GDNF family receptor alpha 3, GFR α-3/GFRA3, GDNF family receptor α3, GDNFR3 |
Construction | The Human GFRa3 (NP_001487.2) (Met 1-Trp 382) was fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus. |
Protein Purity | > 80 % as determined by SDS-PAGE |
分子量 | 67.3 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 3 (GFRA3) or GDNFRa3 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA3 / GDNFRa3 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. The neurotrophic growth factor artemin binds selectively to GDNF family receptor α3 (GFRA3 / GDNFRa3), forming a molecular complex with the co-receptor RET which mediates downstream signaling. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons. |
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Please read the User Guide of Recombinant Proteins for more specific information.
GFR Alpha-3/GFRA3 Protein, Human, Recombinant (His & hFc) GDNF family receptor a 3 GDNF family receptor alpha 3 GFR α-3/GFRA3 GDNF family receptor α3 GDNFR3 GDNFR-3 GDNFR 3 GDNF family receptor α 3 recombinant recombinant-proteins proteins protein