GLO1 Protein, Mouse, Recombinant (His)

カタログ番号 TMPY-01618

別名: glyoxalase I, Glo-1r, GLY1, Glo1-r, 2510049H23Rik, Glo1-s, AW550643, Glo-1, Qglo, Glo-1s, 1110008E19Rik, 0610009E22Rik

Lactoylglutathione lyase, also known as Methylglyoxalase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, S-D-lactoylglutathione methylglyoxal lyase and GLO1, is a member of the glyoxalase I family. GLO1 / Glyoxalase I is a ubiquitous cellular defense enzyme involved in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Accumulative evidence suggests an important role of GLO1 expression in protection against methylglyoxal-dependent protein adduction and cellular damage associated with diabetes, cancer, and chronological aging. GLO1 / Glyoxalase I has been implicated in anxiety-like behavior in mice and in multiple psychiatric diseases in humans. GLO1 / Glyoxalase I catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. GLO1 / Glyoxalase I exists in three separable isoforms which originate from two alleles in the genome. These correspond to two homodimers and one heterodimer composed of two subunits showing different electrophoretic properties. GLO1 upregulation may play a functional role in glycolytic adaptations of cancer cells.

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GLO1 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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参考文献

説明

Species	Mouse
Expression Host	E. coli
Tag	N-His
Accession Number	A5GZX3
別名	glyoxalase I, Glo-1r, GLY1, Glo1-r, 2510049H23Rik, Glo1-s, AW550643, Glo-1, Qglo, Glo-1s, 1110008E19Rik, 0610009E22Rik
Construction	The Mouse GLO1 (NP_079650.3) (Ala 2-Ile 184) was expressed, with a polyhistide tag at the N-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	21.6 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Lactoylglutathione lyase, also known as Methylglyoxalase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, S-D-lactoylglutathione methylglyoxal lyase and GLO1, is a member of the glyoxalase I family. GLO1 / Glyoxalase I is a ubiquitous cellular defense enzyme involved in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Accumulative evidence suggests an important role of GLO1 expression in protection against methylglyoxal-dependent protein adduction and cellular damage associated with diabetes, cancer, and chronological aging. GLO1 / Glyoxalase I has been implicated in anxiety-like behavior in mice and in multiple psychiatric diseases in humans. GLO1 / Glyoxalase I catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. GLO1 / Glyoxalase I exists in three separable isoforms which originate from two alleles in the genome. These correspond to two homodimers and one heterodimer composed of two subunits showing different electrophoretic properties. GLO1 upregulation may play a functional role in glycolytic adaptations of cancer cells.

参考文献

1. Wu, YY. et al., 2008,Prog Neuropsychopharmacol Biol Psychiatry. 32 (7):1740-4. 2. Williams,R. et al., 2009, PLoS One 4 (3): e4649. 3. Antognelli,C. et al., 2009, BMC Cancer 9 :115.Bair,W.B. et al., 2010, Melanoma Res 20 (2):85-96.

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Keywords

GLO1 Protein, Mouse, Recombinant (His) glyoxalase I Glo-1r GLY1 Glo1-r 2510049H23Rik Glo1-s GLY 1 AW550643 Glo-1 Qglo AW-550643 Glo-1s 1110008E19Rik AW 550643 GLY-1 0610009E22Rik recombinant recombinant-proteins proteins protein