GM-CSF/CSF2 Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 14.1 kDa and the accession number is P01587.
パッケージサイズ | 在庫状況 | 単価(税別) |
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20 μg | 在庫あり | ¥ 24,500 |
100 μg | 約5 days | ¥ 68,000 |
200 μg | 約5 days | ¥ 187,500 |
生物学的情報 |
Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 0.01-0.04 ng/mL.
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説明 | GM-CSF/CSF2 Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 14.1 kDa and the accession number is P01587. |
Species | Mouse |
Expression Host | HEK293 Cells |
Tag | Tag Free |
Accession Number | P01587 |
別名 | MGI-IGM, colony stimulating factor 2 (granulocyte-macrophage), GMCSF, Gm-CSf, Csfgm |
Construction | Mouse CSF2 (P01587) (Met1-Lys141) |
Protein Purity |
> 95 % as determined by SDS-PAGE
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分子量 | 14.1 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Granulocyte-macrophage colony-stimulating factor (GM-CSF) is one of an array of cytokines with pivotal roles in embryo implantation and subsequent development. Several cell lineages in the reproductive tract and gestational tissues synthesise GM-CSF under direction by ovarian steroid hormones and signalling agents originating in male seminal fluid and the conceptus. The pre-implantation embryo, invading placental trophoblast cells and the abundant populations of leukocytes controlling maternal immune tolerance are all subject to GM-CSF regulation. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 (cellular) immune responses in cognate T cells. The active form of the protein is found extracellularly as a homodimer, and the encoding gene is localized to a related gene cluster at chromosome region 5q31 which is known to be associated with 5q-syndrome and acute myelogenous leukemia. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant. While the benefits of GM-CSF in this arena have been promising, recent reports have suggested the potential for GM-CSF to induce immune suppression and, thus, negatively impact outcomes in the management of cancer patients. GM-CSF deficiency in pregnancy adversely impacts fetal and placental development, as well as progeny viability and growth after birth, highlighting this cytokine as a central maternal determinant of pregnancy outcome with clinical relevance in human fertility.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
GM-CSF/CSF2 Protein, Mouse, Recombinant MGI-IGM granulocyte-macrophage colony stimulating factor 2 (granulocyte-macrophage) GMCSF colony stimulating factor 2 Gm-CSf Csfgm recombinant recombinant-proteins proteins protein