GP1BB Protein, Mouse, Recombinant (hFc)

GP1BB Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 39.5 kDa and the accession number is P56400.

GP1BB Protein, Mouse, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 107,000
500 μg	約5 days	￥ 424,500
1 mg	約5 days	￥ 711,500

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説明	GP1BB Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 39.5 kDa and the accession number is P56400.

Species	Mouse
Expression Host	HEK293 Cells
Tag	C-hFc
Accession Number	P56400
別名	BDPLT1, GPIb-beta, CD42c, GPIb-β, GP-Ib β, GP-Ib beta, GPIbb, GP1BB, BS, GPIbβ, GPIbbeta
Construction	Pro27-Cys147
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	39.5 kDa (predicted). Due to glycosylation, the protein migrates to 46-50 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The glycoprotein (GP) Ib-IX complex is the receptor on platelet surfaces that mediates their adhesion to subendothelium. It comprises three polypeptides (GP Ib alpha, GP Ib beta, GP IX), each of which belongs to a superfamily of proteins containing conserved leucine-rich motifs. Association between GP Ib alpha and GP Ib beta was demonstrated biochemically on immunoblots of detergent lysates of CHO alpha beta cells; electrophoresis under nonreducing conditions revealed the two subunits to be covalently linked through a disulfide bond.

参考文献

1. López JA, et al. Glycoprotein (GP) Ib beta is the critical subunit linking GP Ib alpha and GP IX in the GP Ib-IX complex. Analysis of partial complexes. J Biol Chem. 1994 Sep 23;269(38):23716-2PMID: 8089142.

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GP1BB Protein, Mouse, Recombinant (hFc)

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