GPRC5D Protein, Human, Recombinant (His & FLAG)

GPRC5D Protein, Human, Recombinant (His & FLAG) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 32.83 kDa and the accession number is Q9NZD1.

GPRC5D Protein, Human, Recombinant (His & FLAG)

パッケージサイズ	在庫状況	単価(税別)
20 μg	約5 days	￥ 555,500
100 μg	約5 days	￥ 1,948,500

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説明	GPRC5D Protein, Human, Recombinant (His & FLAG) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 32.83 kDa and the accession number is Q9NZD1.

Species	Human
Expression Host	HEK293
Tag	His,FLAG
Accession Number	Q9NZD1
別名	GPRC5D
Construction	A DNA sequence encoding the human GPRC5D (Q9NZD1) (Met1-Ser268) was expressed, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Protein Purity	≥ 75 % as determined by SDS-PAGE.
分子量	32.83 kDa (predicted)

Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Formulation	Supplied as solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Stability & Storage	Samples are stable for up to three months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	It is supplied and shipped as Solution with dry ice.
Research Background	Orphan G-protein-coupled receptor family C group 5 member D (GPRC5D) has seven transmembrane segments forming the core of the receptor, as well as an intracellular C-tail and an extracellular N-terminal domain. GPRC5D is highly and selectively expressed on the surface of CD138+ multiple myeloma (MM) cells, whereas in normal tissues, a much lower expression is limited to plasma cells and certain hard keratinizing tissues. This makes GPRC5D a promising marker for monitoring tumor load and a target antigen for MM-specific T cell-engaging therapies such as bispecific antibodies and CAR-T cells.

参考文献

1. Eric L. Smith, et al. GPRC5D is a target for the immunotherapy of multiple myeloma with rationally designed CAR T cells. Sci. Transl. Med. 2019. 2. Christie, et al. Preclinical activity and determinants of response of the GPRC5DxCD3 bispecific antibody talquetamab in multiple myeloma. Blood Adv 2021. 3. Tatsushi Kodama, et al. Anti-GPRC5D/CD3 Bispecific T-Cell–Redirecting Antibody for the Treatment of Multiple Myeloma. Mol Cancer Ther 1 September 2019.

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GPRC5D Protein, Human, Recombinant (His & FLAG)

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