| 説明 |
The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14. |
| Species |
SARS-CoV-2
|
| Expression Host |
E. coli
|
| Tag |
N-6xHis
|
| Accession Number |
YP_009725309.1
|
| 別名 |
SARS-CoV 2 nsp14, Guanine-N7 methyltransferase, SARS-CoV 2 ExoN
|
| Amino Acid |
Ala1-Gln527
|
| Construction |
Ala1-Gln527
|
| 分子量 |
60 KDa (reducing condition)
|
| Formulation |
Supplied as a 0.2 μm filtered solution of 20 mM PB, 100 mM NaCl, 2 mM DTT, 20% Glycerol, 0.1% TritonX-100, pH 6.0.
|
| Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
|
| Research Background |
The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.
|
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