Haptoglobin Protein, Human, Recombinant (C-His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 16 and 40-75 KDa and the accession number is P00738.
| 説明 |
Haptoglobin Protein, Human, Recombinant (C-His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 16 and 40-75 KDa and the accession number is P00738. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-6xHis
|
| Accession Number |
P00738
|
| 別名 |
Zonulin, HP, Haptoglobin
|
| Amino Acid |
Val19-Gln160(alpha
|
| Construction |
Val19-Gln160(alpha chain)&Ile162-Asn406(beta chain)
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. Greater than 72% as determined by SEC-HPLC.
|
| 分子量 |
16&40-75 KDa, reducing conditions
|
| Endotoxin |
< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
|
| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
|
| Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
|
| Research Background |
Haptoglobin(HP) is a secreted protein which is a member of the peptidase S1 family of serine proteases. As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
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