HE4 Protein, Mouse, Recombinant (His)

HE4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 16.4 kDa and the accession number is Q4FZJ6.

HE4 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500
500 μg	約5 days	￥ 750,500

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説明	HE4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 16.4 kDa and the accession number is Q4FZJ6.

Species	Mouse
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	Q4FZJ6
別名	HE4, WAP four-disulfide core domain 2, WAP5, 1600023A02Rik
Construction	The Mouse Wfdc2 (NP_080599.1) (Met1-Phe174) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE.
分子量	16.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2 and HE4, is a secreted protein that contains two WAP domains. WFDC2 / HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. It is highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune defences of the lung, nasal and oral cavities and suggest that WFDC2 functions in concert with related WAP domain containing proteins in epithelial host defence. WFDC2 re-expression in lung carcinomas may prove to be associated with tumour type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation and absent in mid-late lactation. WFDC2 / HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.

参考文献

1. Bingle,L. et al., 2002, Oncogene. 21 (17):2768-73. 2. Hellström,I. et al., 2003, Cancer Res. 63 (13):3695-700. 3. Bingle,L. et al., 2006, Respir Res. 7 : 61. 4. Galgano,M.T. et al., 2006, Mod Pathol.19 (6):847-53. 5. Sharp,J.A. et al., 2007, Evol Dev. 9 (4): 378-92.

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HE4 Protein, Mouse, Recombinant (His)

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