SARS-CoV-2 Helicase Protein (His & MBP)

カタログ番号 TMPJ-01450

別名: SARS-CoV 2 nsp13, SARS-CoV 2 Helicase

The non—structural protein 13 (nsp13) of SARS—CoV 2 is a helicase that separates double—stranded RNA or DNA with a 5'—3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5’-3’ direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom’s syndrome, Werner’s syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

SARS-CoV-2 Helicase Protein (His & MBP)

パッケージサイズ	在庫状況	単価(税別)
10 μg	約5 days	￥ 35,500
50 μg	約5 days	￥ 107,000
500 μg	約5 days	￥ 569,500
1 mg	約5 days	￥ 854,000

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生物学的特性に関する説明

Technical Params

Product Properties

説明

Species	SARS-CoV-2
Expression Host	E. coli
Tag	C-6xHis
Accession Number	P0DTD1
別名	SARS-CoV 2 nsp13, SARS-CoV 2 Helicase
Amino Acid	Ala5325-Gln5925
Construction	Ala5325-Gln5925
Protein Purity	Greater than 80% as determined by reducing SDS-PAGE. (QC verified)
分子量	120 KDa (reducing condition)

Formulation	Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	The non—structural protein 13 (nsp13) of SARS—CoV 2 is a helicase that separates double—stranded RNA or DNA with a 5'—3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5’-3’ direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom’s syndrome, Werner’s syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.

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Keywords

SARS-CoV-2 Helicase Protein (His & MBP) SARS-CoV 2 nsp13 SARS-CoV 2 Helicase recombinant recombinant-proteins proteins protein