Hemojuvelin Protein, Cynomolgus, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 40.2 kDa and the accession number is A0A2K5TJX5.
説明 | Hemojuvelin Protein, Cynomolgus, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 40.2 kDa and the accession number is A0A2K5TJX5. |
Species | Cynomolgus |
Expression Host | Baculovirus Insect Cells |
Tag | C-His |
Accession Number | A0A2K5TJX5 |
別名 | hemochromatosis type 2 (juvenile) |
Construction | The cynomolgus HFE2 (EHH15137.1) (Met1-Ser400) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity | > 95 % as determined by SDS-PAGE |
分子量 | 40.2 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Hemojuvelin, also known as HFE2, is a membrane-bound and soluble protein that belongs to the repulsive guidance molecule (RGM) family. It is known that RGMs function through Neogenin, a homolog of the Netrin receptor deleted in colon cancer. In mammals, the RGM family consists of three glycoproteins that have discrete expression patterns and functions (RGM-A, RGM-B, and RGM-C). Hemojuvelin is expressed in adult and fetal liver, heart, and skeletal muscle. Hemojuvelin acts as a bone morphogenetic protein (BMP) coreceptor. Enhancement of BMP signaling regulates hepcidin (HAMP) expression and iron metabolism. It plays a key role in iron metabolism. Hemojuvelin represents the cellular receptor for hepcidin. It may be a component of the signaling pathway that activates hepcidin or it may act as a modulator of hepcidin expression. Defects in hemojuvelin are the cause of hemochromatosis type 2A, also known as juvenile hemochromatosis (JH). |
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Please read the User Guide of Recombinant Proteins for more specific information.
Hemojuvelin Protein, Cynomolgus, Recombinant (His) hemochromatosis type 2 (juvenile) juvenile hemochromatosis type 2 recombinant recombinant-proteins proteins protein