HER2/ERBB2 Protein, Human, Recombinant (aa 676-1255, His & GST)

HER2/ERBB2 Protein, Human, Recombinant (aa 676-1255, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 92 kDa and the accession number is P04626-1.

HER2/ERBB2 Protein, Human, Recombinant (aa 676-1255, His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 91,500

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説明	HER2/ERBB2 Protein, Human, Recombinant (aa 676-1255, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 92 kDa and the accession number is P04626-1.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	P04626-1
別名	TKR1, HERV-7q, ERVWE1, EGFR2, HERV7Q, HERVW, MLN19, HERVWENV, HER2, MLN 19, NGL, NEU, ENV, CD340, erb-b2 receptor tyrosine kinase 2, HER-2/neu, HERV-W-ENV, ENVW, HER-2
Construction	The cytoplasmic domain (Lys 676-Val 1255) of Human ErbB2 (NP_004439.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	92 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10% gly, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Krawczyk N,et al.(2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.

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HER2/ERBB2 Protein, Human, Recombinant (aa 676-1255, His & GST)

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