HER2/ERBB2 Protein, Human, Recombinant , Biotinylated

HER2/ERBB2 Protein, Human, Recombinant , Biotinylated is expressed in HEK293 mammalian cells. The predicted molecular weight is 70 kDa and the accession number is P04626-1.

HER2/ERBB2 Protein, Human, Recombinant , Biotinylated

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 45,500
100 μg	約5 days	￥ 107,500
200 μg	約5 days	￥ 183,000
500 μg	約5 days	￥ 369,500

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生物学的情報	1. Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD), Biotinylated at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 10-50 ng/mL. 2. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant, Biotinylated bound to Pertuzumab was 0.1 nM.
説明	HER2/ERBB2 Protein, Human, Recombinant , Biotinylated is expressed in HEK293 mammalian cells. The predicted molecular weight is 70 kDa and the accession number is P04626-1.

Species	Human
Expression Host	HEK293 Cells
Tag	Tag Free
Accession Number	P04626-1
別名	CD340, ENVW, ENV, HER2, HERV7Q, HERVWENV, HERV-7q, HER-2, TKR1, EGFR2, HERVW, NGL, NEU, erb-b2 receptor tyrosine kinase 2, MLN 19, HER-2/neu, HERV-W-ENV, ERVWE1, MLN19
Construction	The Human ERBB2 (NP_004439.2) (Met1-Thr652) was expressed with five amino acids (DDDDK) at the C-terminus. The purified protein was biotinylated in vitro.
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	70 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Krawczyk N,et al.(2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.

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HER2/ERBB2 Protein, Human, Recombinant , Biotinylated

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