HER2/ERBB2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 70 kDa and the accession number is P04626-1.
パッケージサイズ | 在庫状況 | 単価(税別) |
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100 μg | 在庫あり | ¥ 116,000 |
200 μg | 約5 days | ¥ 204,000 |
500 μg | 約5 days | ¥ 431,500 |
生物学的情報 |
1. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.3-1.5 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
2. Immobilized Human Her2/ERBB2 at 2 μg/ml (100 μl/well) can bind Herceptin, the EC50 of Herceptin is 7.0-30.0 ng/mL.
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説明 | HER2/ERBB2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 70 kDa and the accession number is P04626-1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | Tag Free |
Accession Number | P04626-1 |
別名 | HER-2/neu, erb-b2 receptor tyrosine kinase 2, HER2, HERV7Q, HERVWENV, NEU, CD340, NGL, EGFR2, MLN19, HERV-W-ENV, TKR1, ERVWE1, HERV-7q, MLN 19, HERVW, HER-2, ENV, ENVW |
Construction | The Human ERBB2 (NP_004439.2) (Met1-Thr652) was expressed with five amino acids (DDDDK) at the C-terminus. |
Protein Purity |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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分子量 | 70 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
HER2/ERBB2 Protein, Human, Recombinant HER 2 HER-2/neu erb-b2 receptor tyrosine kinase 2 MLN-19 HER2 ERVWE 1 HERV7Q HERVWENV NEU TKR-1 CD340 CD 340 CD-340 NGL TKR 1 EGFR2 MLN19 HERV-W-ENV TKR1 ERVWE1 HERV-7q MLN 19 HERVW HER-2 ENV ERVWE-1 ENVW recombinant recombinant-proteins proteins protein