HER3/ERBB3 Protein, Human, Recombinant (aa 730-1065, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 65 kDa and the accession number is P21860-1.
説明 | HER3/ERBB3 Protein, Human, Recombinant (aa 730-1065, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 65 kDa and the accession number is P21860-1. |
Species | Human |
Expression Host | Baculovirus Insect Cells |
Tag | His, GST |
Accession Number | P21860-1 |
別名 | c-erbB3, ErbB-3, p45-sErbB3, LCCS2, p85-sErbB3, HER3, EEBB3, MDA-BF-1, p180-ErbB3, c-erbB-3, erbB3-S, erb-b2 receptor tyrosine kinase 3 |
Construction | The cytoplasmic domain (Pro 730-Ser 1065) of Human ErbB3 (NP_001973.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Protein Purity | > 75 % as determined by SDS-PAGE |
分子量 | 65 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, pH 7.5, 10% gly, 1 mM GSH. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
HER3/ERBB3 Protein, Human, Recombinant (aa 730-1065, His & GST) c-erbB3 ErbB-3 EEBB-3 p45-sErbB3 LCCS 2 LCCS-2 EEBB 3 LCCS2 p85-sErbB3 HER3 EEBB3 HER-3 MDA-BF-1 p180-ErbB3 c-erbB-3 HER 3 erbB3-S erb-b2 receptor tyrosine kinase 3 recombinant recombinant-proteins proteins protein