HER3/ERBB3 Protein, Human, Recombinant (His), Biotinylated

HER3/ERBB3 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 70.2 kDa and the accession number is P21860-1.

HER3/ERBB3 Protein, Human, Recombinant (His), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 59,000
100 μg	約5 days	￥ 135,500
200 μg	約5 days	￥ 229,500
500 μg	約5 days	￥ 466,000

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生物学的情報	Immobilized Recombinant Human NRG1 / Neuregulin 1 Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human HER3 / ErbB3 Protein (His Tag), Biotinylated , the EC50 is 100-300 ng/mL.
説明	HER3/ERBB3 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 70.2 kDa and the accession number is P21860-1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P21860-1
別名	MDA-BF-1, c-erbB-3, HER3, p85-sErbB3, LCCS2, p180-ErbB3, erbB3-S, erb-b2 receptor tyrosine kinase 3, EEBB3, p45-sErbB3, ErbB-3, c-erbB3
Construction	The extracellular domain (Met1-Thr643) of Human ErbB3 (NP_001973.2) precursor was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
Protein Purity	> 95% as determined by SDS-PAGE.
分子量	70.2 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Kraus M.H.,et al.,(1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197. 2. Plowman G.D.,et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909. 3. Katoh M.,et al.,(1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.

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HER3/ERBB3 Protein, Human, Recombinant (His), Biotinylated

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