HGF Protein, Mouse, Recombinant

HGF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 79.3 kDa and the accession number is Q08048-1.

HGF Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 31,500
100 μg	約5 days	￥ 126,000
200 μg	約5 days	￥ 202,000
500 μg	約5 days	￥ 376,500

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生物学的情報	1. Immobilized mouse HGF at 10 μg/ml (100 μl/well) can bind mouse MET-Fc , EC50 of mouse MET-Fc is 0.05-0.12 μg/ml. 2. Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 1-4 ng/ml.
説明	HGF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 79.3 kDa and the accession number is Q08048-1.

Species	Mouse
Expression Host	HEK293 Cells
Tag	Tag Free
Accession Number	Q08048-1
別名	hepatocyte growth factor (hepapoietin A, NK1, scatter factor), SF, C230052L06Rik, SF/HGF, HGF/SF, NK2
Construction	The Mouse HGF (Q08048-1) (Met1-Leu728) was expressed and purified.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	79.3 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain, and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. The hepatocyte growth factor is secreted by mesenchymal cells as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility and matrix invasion give it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Naldini L,et al.(1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 10(10):2867-78. 2. Comoglio,et al.(1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65. 3. HAhn W,et al.(2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55. 4. Bottaro DP,et al.(1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.

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HGF Protein, Mouse, Recombinant

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