HIST1H3A Protein, Human, Mouse, Recombinant

カタログ番号 TMPY-01332

別名: histone cluster 1, H3a

Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of the histone H3 family which is a core component of nucleosome. It is expressed during the S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher-order chromatin structures.

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HIST1H3A Protein, Human, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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生物学的特性に関する説明

Technical Params

Product Properties

参考文献

説明

Species	Human,Mouse
Expression Host	E. coli
Tag	Tag Free
Accession Number	P68431
別名	histone cluster 1, H3a
Construction	The native Human / Mouse HIST1H3A (NP_003520.1) (Met1-Ala136) was expressed. Human and Mouse HIST1H3A sequences are identical.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	15.5 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 2 mM β-Mercaptoethanol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of the histone H3 family which is a core component of nucleosome. It is expressed during the S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher-order chromatin structures.

参考文献

1. Lachner M, et al., 2001, Nature 410 (6824): 116-20. 2. Koessler H, et al., 2003, DNA Cell Biol. 22 (4): 233-41. 3. Macdonald N. et al., 2005, Mol. Cell 20: 199-211. 4. Hyllus D. et al., 2007, Genes Dev. 21: 3369-3380. 5. Garcia BA. et al., 2007, J. Biol. Chem. 282:7641-7655. 6. Yu L.-R. et al., 2007, J. Proteome Res. 6: 4150-4162.

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Keywords

HIST1H3A Protein, Human, Mouse, Recombinant histone cluster 1, H3a recombinant recombinant-proteins proteins protein