HK2 Protein, Mouse, Recombinant (His)

HK2 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 103.99 kDa and the accession number is O08528.

HK2 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 91,500

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説明	HK2 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 103.99 kDa and the accession number is O08528.

Species	Mouse
Expression Host	Baculovirus Insect Cells
Tag	C-His
Accession Number	O08528
Construction	The Mouse HK2 (O08528) (Met1-Arg917) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
分子量	103.99 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 300 mM NaCl, 10% glycerol, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of the glycolysis pathway, expresses at a high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic processes. The glycolytic enzyme hexokinase 2 (HK2) is crucial for the Warburg effect in human glioma, the most common malignant brain tumor. Although absent in most adult tissues, hexokinase 2 (HK2) is expressed in a majority of tumors and contributes to increased glucose consumption and to in vivo tumor 18F-FDG PET signaling. Hexokinase 2 (HK2) is a rate-determining enzyme in aerobic glycolysis, a process upregulated in tumor cells. HK2 expression is controlled by various transcription factors and epigenetic alterations and is heterogeneous in hepatocellular carcinomas (HCCs), though the cause of this heterogeneity is not known.

参考文献

1. Xu S,et al.(2018) Hexokinase 2 is targetable for hk1 negative, hk2 positive tumors from a wide variety of tissues of origin. J Nucl MedLiu H,et al.(2017) Hexokinase 2 (hk2), the tumor promoter in glioma, is downregulated by mir-218/bmi1 pathway. PLoS One 12 (12): e0189353. 2. Lee HG,et al.(2016) Regulation of hk2 expression through alterations in cpg methylation of the hk2 promoter during progression of hepatocellular carcinoma. Oncotarget 7 (27): 41798-41810. 3. Bao F,et al.(2018) New natural inhibitors of hexokinase 2 (hk2): Steroids from ganoderma sinense. Fitoterapia 125 123-129.

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HK2 Protein, Mouse, Recombinant (His)

参考文献

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