Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.
説明 | Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4. |
Species | Human |
Expression Host | HEK293 |
Tag | C-His-Avi |
Accession Number | A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV |
別名 | MAGE4B, MAGE-A4, member 4, CT1.4, HLA0201, MAGE4A, MAGE-X2, MHC I |
Construction | Recombinant Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and GVYDGREHTV peptide.[Accession |A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV] |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt.-20 to -80°C for 3-6 months in unopened state after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4. |
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Please read the User Guide of Recombinant Proteins for more specific information.
HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein, Human, MHC (His & Avi) MAGE4B MAGE-A4 member 4 CT1.4 HLA0201 MAGE4A MAGE-X2 MHC I recombinant recombinant-proteins proteins protein