HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein, Human, MHC (His & Avi)

PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein, Human, MHC (His & Avi)

パッケージサイズ	在庫状況	単価(税別)
100 μg	お問い合わせ	￥ 33,500
1 mg	お問い合わせ	￥ 225,000

バルクのお問い合わせ

説明	PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His-Avi
Accession Number	P79603(HLA-A*02:01)&P61769(B2M)&ALYVDSLFFL
別名	OIP-4, PRAME, MAPE, OIP4
Construction	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 100 mM Arginine (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

参考文献

1. Lezcano C, et al. PRAME Immunohistochemistry as an Ancillary Test for the Assessment of Melanocytic Lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001. Epub 2021 Apr 28. PMID: 34023098; PMCID: PMC8152939.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein, Human, MHC (His & Avi)

参考文献

Calculator

技術サポート

Keywords