Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
説明 | Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail. |
Species | Human |
Expression Host | HEK293 |
Tag | C-His-Avi |
Accession Number | NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVVGAVGVGK |
別名 | KRAS, NS3, GTPase Kras, RASK2, K-RAS2B, C-K-RAS, MHC, KRAS2, KI-RAS, KRAS1, K-Ras 2, NS, K-RAS4A, RALD, CFC2, K-RAS2A, K-RAS4B |
Construction | Recombinant Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide.[Accession |NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVVGAVGVGK] |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt.-20 to -80°C for 3-6 months in unopened state after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail. |
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Please read the User Guide of Recombinant Proteins for more specific information.
HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein, Human, MHC (His & Avi) KRAS NS3 GTPase Kras RASK2 K-RAS2B C-K-RAS MHC KRAS2 KI-RAS KRAS1 K-Ras 2 NS K-RAS4A RALD CFC2 K-RAS2A K-RAS4B recombinant recombinant-proteins proteins protein