HMGN3 Protein, Human, Recombinant (His)

カタログ番号 TMPY-02979

別名: PNAS-24, TRIP7, PNAS-25, high mobility group nucleosomal binding domain 3

HMGN3 belongs to the HMGN family and is expressed in the kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate, and uterus. Members of the HMGN family bind to nucleosomes without any specificity for the underlying DNA sequence. They affect the global and local structure of chromatin, as well as the levels of histone modifications, and thus play a role in epigenetic regulation of gene expression. HMGN3 regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. It also regulates the expression of SLC6A9, a glycine transporter that regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. Both insulin and glucagon levels can be affected by HMGN3. HMGN3 may play a role in ocular development and astrocyte function. It also modulates the expression of pancreatic genes involved in insulin secretion.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

HMGN3 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

バルクのお問い合わせ

DATASHEET SDS

生物学的特性に関する説明

Technical Params

Product Properties

参考文献

説明

Species	Human
Expression Host	E. coli
Tag	N-His
Accession Number	Q15651-2
別名	PNAS-24, TRIP7, PNAS-25, high mobility group nucleosomal binding domain 3
Construction	mature form of Human HMGN3 (Q15651-2) (Met1-Asn77)
Protein Purity	> 96 % as determined by SDS-PAGE
分子量	10.2 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 50 mM Arg, 10% glycerol, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	HMGN3 belongs to the HMGN family and is expressed in the kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate, and uterus. Members of the HMGN family bind to nucleosomes without any specificity for the underlying DNA sequence. They affect the global and local structure of chromatin, as well as the levels of histone modifications, and thus play a role in epigenetic regulation of gene expression. HMGN3 regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. It also regulates the expression of SLC6A9, a glycine transporter that regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. Both insulin and glucagon levels can be affected by HMGN3. HMGN3 may play a role in ocular development and astrocyte function. It also modulates the expression of pancreatic genes involved in insulin secretion.

参考文献

1. Ueda T,et al.(2009) The nucleosome binding protein HMGN3 modulates the transcription profile of pancreatic beta cells and affects insulin secretion. Mol Cell Biol. 29(19):5264-76. 2. Lei SF,et al.(2012) Genome-wide association study identifies HMGN3 locus for spine bone size variation in Chinese. Hum Genet. 131(3):463-9. 3. Kim W,et al.(2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

技術サポート

Please read the User Guide of Recombinant Proteins for more specific information.

Keywords

HMGN3 Protein, Human, Recombinant (His) PNAS 24 PNAS-24 TRIP7 PNAS-25 PNAS24 PNAS25 high mobility group nucleosomal binding domain 3 TRIP 7 TRIP-7 PNAS 25 recombinant recombinant-proteins proteins protein