HSP90 alpha Protein, Human, Recombinant

HSP90 alpha Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 22.6 kDa and the accession number is K9JA46.

HSP90 alpha Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 156,500
500 μg	約5 days	￥ 330,500

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生物学的情報	Testing in progress
説明	HSP90 alpha Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 22.6 kDa and the accession number is K9JA46.

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	K9JA46
別名	Hsp89, Hsp90, HSPCAL1, heat shock protein 90kDa alpha (cytosolic), class A member 1, HSPN, heat shock protein 90kDa α (cytosolic), class A member 1, HSP90N, LAP2, HSPCAL4, Hsp90 α, HSP90A, HSP86, HSP89A, HSPCA, HSPC1, LAP-2, EL52
Construction	A DNA sequence encoding the human HSP90 isoform 2 (NP_005339.3) C-terminal segment, corresponding to amino acid sequence (Glu 535-Asp 732) was expressed and purified, with two additonal aa (Gly & Pro) at the N terminus.
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	22.6 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Heat shock protein 90 (90 kDa heat-shock protein, HSP90) is a molecular chaperone involved in the trafficking of proteins in the cell. It is a remarkably versatile protein involved in the stress response and normal homoeostatic control mechanisms. HSP90 interacts with 'client proteins', including protein kinases, transcription factors, and others, and either facilitates their stabilization and activation or directs them for proteasomal degradation. By this means, HSP90 displays a multifaceted ability to influence signal transduction, chromatin remodeling and epigenetic regulation, development, and morphological evolution. HSP90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis at the N-terminus. Disruption of HSP90 leads to client protein degradation and often cell death. Under stressful conditions, HSP90 stabilizes its client proteins and protects the cell against cellular stressors such as in cancer cells. Especially, several oncoproteins act as HSP90 client proteins and tumor cells require higher HSP90 activity than normal cells to maintain their malignancy. For this reason, Hsp90 has emerged as a promising target for anti-cancer drug development.

参考文献

1. Pearl LH,et al.(2008) The Hsp90 molecular chaperone: an open and shut case for treatment. Biochem J. 410(3): 439-53. 2. Hahn JS. (2009) The Hsp90 chaperone machinery: from structure to drug development. BMB Rep. 42(10): 623-30. 3. Holzbeierlein JM,et al.(2010) Hsp90: a drug target? Curr Oncol Rep. 12(2): 95-101. 4. Trepel J,et al.(2010) Targeting the dynamic HSP90 complex in cancer. Nat Rev Cancer. 10(8): 537-49.

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HSP90 alpha Protein, Human, Recombinant

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