HSV 2 (strain 333) Glycoprotein B/gB Protein (His)

HSV 2 (strain 333) Glycoprotein B/gB Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 73.88 kDa and the accession number is P06763.

HSV 2 (strain 333) Glycoprotein B/gB Protein (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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説明	HSV 2 (strain 333) Glycoprotein B/gB Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 73.88 kDa and the accession number is P06763.

Species	HSV2
Expression Host	Baculovirus Insect Cells
Tag	C-His
Accession Number	P06763
Construction	The HSV 2 (strain 333 (HSV2)) gB (translated amino acids of P06763) (Glu98-Ala730) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE.
分子量	73.88 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 200 mM NaCl, 20% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.

参考文献

1. Lopper M,et al.(2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82. 2. Isaacson MK,et al.(2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.

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HSV 2 (strain 333) Glycoprotein B/gB Protein (His)

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