Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (aa 1-700, hFc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 104 kDa and the accession number is P13201.
説明 | Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (aa 1-700, hFc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 104 kDa and the accession number is P13201. |
Species | CMV |
Expression Host | HEK293 Cells |
Tag | Fc |
Accession Number | P13201 |
別名 | Human cytωlovirus (HCMV) Glycoprotein B/gB |
Construction | The extracellular domain (Met 1-Lys 700) of Human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') precursor was fused with the Fc region of Human IgG1 at the C-terminus. |
Protein Purity | > 80 % as determined by SDS-PAGE |
分子量 | 104 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium. |
bottom
Please read the User Guide of Recombinant Proteins for more specific information.
Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (aa 1-700, hFc) Human cytωlovirus (HCMV) Glycoprotein B/gB recombinant recombinant-proteins proteins protein