PCSK9 Protein, Human, Recombinant (D374Y & V474I & G670E, His)

PCSK9 Protein, Human, Recombinant (D374Y & V474I & G670E, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 72.6 kDa and the accession number is AAV67948.1.

PCSK9 Protein, Human, Recombinant (D374Y & V474I & G670E, His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 88,500
100 μg	約5 days	￥ 138,500
200 μg	約5 days	￥ 215,500
500 μg	約5 days	￥ 388,000

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生物学的情報

1.Measured by its binding ability in a functional ELISA. Immobilized human PCSK9-His(D374Y)at 10 μg/mL (100 μL /well) can bind biotinylated LDLR-His , The EC50 of biotinylated LDLR-His is 0.2-1.4μg/mL. 2.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag on ProA Biosensor, can bind Human PCSK9 / NARC1 (D374Y, V474I, G670E) Protein, His Tag with an affinity constant of 3.08nM as determined in BLI assay (Routinely tested).

説明

PCSK9 Protein, Human, Recombinant (D374Y & V474I & G670E, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 72.6 kDa and the accession number is AAV67948.1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	AAV67948.1
別名	FH3, proprotein convertase subtilisin/kexin type 9, LDLCQ1, HCHOLA3, NARC1, PC9, NARC-1
Construction	The Human PCSK9 (NP_777596.2, with mutation Asp 374 Tyr, natural mutation Val 474 Ile and Gly 670 Glu) (Met 1-Gln 692) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	72.6 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 100 mM NaCl, 10% Glycerol, 0.01% Tween20, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

参考文献

1. Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933. 2. Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498 3. Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73. 4. Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875. 5. Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.

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PCSK9 Protein, Human, Recombinant (D374Y & V474I & G670E, His)

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