ICAM-1/CD54 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 51 kDa and the accession number is A0A384MEK5.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
50 μg | 在庫あり | ¥ 80,500 |
100 μg | 約5 days | ¥ 121,000 |
200 μg | 約5 days | ¥ 180,500 |
500 μg | 約5 days | ¥ 305,500 |
生物学的情報 | Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM1-coated plates (12.5 μg/ml, 100 μl/well), approximately 30%-60% will adhere specifically. |
説明 | ICAM-1/CD54 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 51 kDa and the accession number is A0A384MEK5. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | His |
Accession Number | A0A384MEK5 |
別名 | CD54, intercellular adhesion molecule 1, BB2, ICAM-1, P3.58 |
Construction | The Human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480) was fused with the a polyhistidine tag at the C-terminus. |
Protein Purity |
≥ 98 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC.
|
分子量 | 51 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
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Please read the User Guide of Recombinant Proteins for more specific information.
ICAM-1/CD54 Protein, Human, Recombinant (His) CD54 intercellular adhesion molecule 1 BB2 BB-2 ICAM1 ICAM-1 CD-54 BB 2 CD 54 P3.58 ICAM 1 recombinant recombinant-proteins proteins protein