IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. IFN-alpha 1/IFNA1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 46.14 kDa and the accession number is P01562.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 約5 days | ¥ 112,000 |
500 μg | 約5 days | ¥ 447,500 |
1 mg | 約5 days | ¥ 746,000 |
説明 | IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. IFN-alpha 1/IFNA1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 46.14 kDa and the accession number is P01562. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-hFc |
Accession Number | P01562 |
別名 | IFN-α-1/13, IFN α1, IFN alpha1, IFNA1, IFN α 1, IFNalpha 1, Interferon alpha 1, Interferon alpha-1/13, Interferon α1, Interferon α-D, Interferon α-1/13, LeIF D, IFN-alpha-1, IFNα1, IFN-α-1, Interferon alpha-D, IFN-alpha-1/13 |
Construction | Cys24-Glu189 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 46.14 kDa (predicted). Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IFN-alpha 1/IFNA1 Protein, Human, Recombinant (hFc) IFN-α-1/13 IFNA 1 IFN α1 Interferon a 1 IFN alpha1 IFNA1 IFN α 1 IFNalpha 1 IFN-a-1/13 Interferon alpha 1 Interferon alpha-1/13 IFN-a-1 Interferon α1 Interferon a-D Interferon α-D IFNA-1 Interferon α-1/13 LeIF D IFN-alpha-1 IFNα1 IFN-α-1 Interferon α 1 Interferon alpha-D Interferon a-1/13 IFN-alpha-1/13 IFNα 1 recombinant recombinant-proteins proteins protein