IFN-alpha 1/IFNA1 Protein, Mouse, Recombinant (hFc)

IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. IFN-alpha 1/IFNA1 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 45.88 kDa and the accession number is P01572.

IFN-alpha 1/IFNA1 Protein, Mouse, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 112,000
500 μg	約5 days	￥ 447,500
1 mg	約5 days	￥ 746,000

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説明

Species	Mouse
Expression Host	HEK293 Cells
Tag	C-hFc
Accession Number	P01572
別名	IFN-α-1/13, IFN alpha1, Interferon α-1/13, Interferon alpha-1/13, IFNalpha 1, Interferon α-D, IFNA1, IFNα1, IFN-alpha-1, IFN α1, LeIF D, IFN α 1, IFN-α-1, Interferon α1, IFN-alpha-1/13, Interferon alpha 1, Interferon alpha-D
Construction	Cys24-Lys189
Protein Purity	> 95% as determined by Tris-Bis PAGE
分子量	45.88 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.

参考文献

1. Coomans de Brachène A, et al. IFN-α induces a preferential long-lasting expression of MHC class I in human pancreatic beta cells. Diabetologia. 2018 Mar;61(3):636-640. doi: 10.1007/s00125-017-4536-4. Epub 2018 Jan 5. PMID: 29305625; PMCID: PMC6241216.

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IFN-alpha 1/IFNA1 Protein, Mouse, Recombinant (hFc)

参考文献

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