Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. IFN gamma Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 19.1 kDa and the accession number is P01579.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 在庫あり | ¥ 42,500 |
500 μg | 約5 days | ¥ 171,000 |
1 mg | 約5 days | ¥ 284,500 |
生物学的情報 | Immobilized Human IFN gamma, His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 32.7 ng/mL determined by ELISA. |
説明 | Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. IFN gamma Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 19.1 kDa and the accession number is P01579. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His-Avi |
Accession Number | P01579 |
別名 | IFN gamma, interferon, Interferon-γ, γ, IFI, Interferon-gamma, IFG, IFN γ/IFNG, gamma, IFNγ, IFN γ |
Construction | Gln24-Gly161 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 19.1 kDa (predicted). Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IFN gamma Protein, Human, Recombinant (His & Avi) IFN gamma interferon Interferon-γ γ Interferon-r IFI Interferon-gamma IFG IFN γ/IFNG IFNgamma gamma IFNγ IFN γ IFNr recombinant recombinant-proteins proteins protein