IFN gamma Protein, Mouse, Recombinant

IFN gamma Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.5 kDa and the accession number is A0A7R8C347.

IFN gamma Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 13,000
100 μg	約5 days	￥ 29,000

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生物学的情報	1. Measured by its binding ability in a functional ELISA. Immobilized Mouse Interferon Gamma at 2 μg/ml (100 μl/well) can bind Mouse IFNGR1 hFc , the EC50 of Mouse IFNGR1 hFc is7.0-30.0 ng/mL. 2. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.05-0.3 ng/mL.
説明	IFN gamma Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.5 kDa and the accession number is A0A7R8C347.

Species	Mouse
Expression Host	HEK293
Tag	Tag Free
Accession Number	A0A7R8C347
別名	IFN γ, interferon, γ, interferon, gamma, Ifg, IFN-g
Construction	A DNA sequence encoding the mouse IFNG (NP_032363.1) (Met1-Cys155) was expressed and purified.
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	15.5 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Gray P W, et al. (1982) Structure of the human immune interferon gene. Nature. 298: 859-63. 2. Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8. 3. Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7. 4. Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36. 5. Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7. 6. Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.

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IFN gamma Protein, Mouse, Recombinant

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