IFN gamma Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.5 kDa and the accession number is P01581.
生物学的情報 | 1. Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 1-5 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized rat IFNG-Fc at 10 μg/ml (100 μl/well) can bind biotinylated rat IFNGR-Fc . The EC50 of biotinylated rat IFNGR-Fc is 20.1-46.9 ng/ml. |
説明 | IFN gamma Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.5 kDa and the accession number is P01581. |
Species | Rat |
Expression Host | HEK293 Cells |
Tag | hFc |
Accession Number | P01581 |
別名 | interferon, γ, interferon, gamma, IFN γ |
Construction | The Rat IFNG (P01581) (Met 1-Cys 156) was fused with the Fc region of Human IgG1 at the C-terminus. |
Protein Purity | > 92 % as determined by SDS-PAGE |
分子量 | 42.5 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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IFN gamma Protein, Rat, Recombinant (hFc) interferon, γ interferon, gamma IFN γ recombinant recombinant-proteins proteins protein