IFN-omega Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.6 kDa and the accession number is P05000.
生物学的情報 |
Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV). The ED50 for this effect is typically 2-8 pg/mL.
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説明 | IFN-omega Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.6 kDa and the accession number is P05000. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His |
Accession Number | P05000 |
別名 | IFN-ω, IFN-ω 1, interferon omega-1, IFNW1, interferon ω-1, interferon, omega 1, IFN-omega 1, interferon, ω 1 |
Construction | The Human IFNW1 (P05000) (Met1-Ser195) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity |
> 95 % as determined by SDS-PAGE
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分子量 | 21.6 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, 10% Glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | IFNs are a large family of proteins having antiviral, antiproliferative, and immunomodulatory effects, and are divided into two major classes, type I and type II, based on differences in receptor binding and nucleotide sequence. Type I IFNs consist of IFN α, β, τ, and ω and bind to the type I IFN receptor, whereas IFN-γ is the only type II IFN and is specific for the type II IFN receptor. Human IFN-ω, was identified by three independent groups in 1985 and is structurally related to IFN-α and -β. Both human IFN-ω and IFN-α are produced by virally induced leukocytes and have similar antiviral activities on human cell lines, and a sizeable proportion (at least 1%) of the total antiviral activity of leukocyte IFN is contributed by IFN-ωl. Also, it was reported that IFN-ω could inhibit the growth of human tumors in vivo. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IFN-omega Protein, Human, Recombinant (His) IFN-ω IFN-ω 1 IFNW-1 interferon omega-1 interferon w-1 IFNW1 interferon ω-1 interferon, w 1 interferon, omega 1 IFNW 1 IFN-omega 1 interferon, ω 1 IFN-w 1 recombinant recombinant-proteins proteins protein