IGF1/IGF-I Protein, Mouse, Recombinant

IGF1/IGF-I Protein, Mouse, Recombinant is expressed in yeast. The predicted molecular weight is 7.7 kDa and the accession number is E9PU89.

IGF1/IGF-I Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 49,000
1 mg	約5 days	￥ 498,000

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生物学的情報	Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 0.2-1.2 μg/mL.
説明	IGF1/IGF-I Protein, Mouse, Recombinant is expressed in yeast. The predicted molecular weight is 7.7 kDa and the accession number is E9PU89.

Species	Mouse
Expression Host	P. pastoris (Yeast)
Tag	Tag Free
Accession Number	E9PU89
別名	insulin-like growth factor 1 (somatomedin C), Igf-I, C730016P09Rik, Igf-1
Construction	Mouse IGF1 (NP_001104744.1) (Gly49-Ala118)
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	7.7 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	IGF I, also known as Mechano Growth Factor, somatomedin-C, IGF-I, and IGF1, is a secreted protein that belongs to the insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ), and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also about enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

参考文献

1. Jansen M., et al.,(1983), Sequence of cDNA encoding human insulin-like growth factor I precursor. Nature 306:609-611. 2. de Pagter-Holthuizen P., et al., (1986), Organization of the human genes for insulin-like growth factors I and II.FEBS Lett. 195:179-184. 3. le Bouc Y., et al.,(1986), Complete characterization of the human IGF-I nucleotide sequence isolated from a newly constructed adult liver cDNA library.FEBS Lett. 196:108-112.

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IGF1/IGF-I Protein, Mouse, Recombinant

参考文献

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