IGF1R/CD221 Protein, Human, Recombinant (His)

IGF1R/CD221 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 81 kDa and the accession number is P08069.

IGF1R/CD221 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 79,500
200 μg	約5 days	￥ 140,500
500 μg	約5 days	￥ 296,000

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生物学的情報

1. Immobilized Anti-IGF-1R Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Human IGF1R / CD221 Protein (His Tag) , the EC50 is 1.2-3.6 ng/mL(QC tested). 2.Captured IGF1R on NTA Chip can bind IGF1 with an affinity constant of 0.1317 µM as determined in a SPR assay (Routinely tested). 3.Loaded Anti-IGF-1R Antibody, human IgG1 on ProA Biosensor, can bind Recombinant Human IGF1R Protein, His Tag with an affinity constant of 0.808nM as determined in BLI assay (Routinely tested).

説明

IGF1R/CD221 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 81 kDa and the accession number is P08069.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P08069
別名	IGF-I R, IGFR, IGFIR, IGF1 Receptor, JTK13, insulin-like growth factor 1 receptor, CD221
Construction	extracellular domain (Met1-Asn932) of Human IGF1R (NP_000866.1)
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	81 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The insulin-like growth factor-1 receptor (IGF1R) is a transmembrane tyrosine kinase involved in several biological processes including cell proliferation, differentiation, DNA repair, and cell survival. This a disulfide-linked heterotetrameric transmembrane protein consisting of two α and two β subunits, and among which, the α subunit is extracellular while the β subunit has an extracellular domain, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. The IGF1R signaling pathway is activated in the mammalian nervous system from the early developmental stages. Its major effect on developing neural cells is to promote their growth and survival. This pathway can integrate its action with signaling pathways of growth and morphogenetic factors that induce cell fate specification and selective expansion of specified neural cell subsets. Modulation of cell migration is another possible role that IGF1R activation may play in neurogenesis. In the mature brain, IGF-I binding sites have been found in different regions of the brain, and multiple reports confirmed a strong neuroprotective action of the IGF-IR against different pro-apoptotic insults. IGF1R is an important signaling molecule in cancer cells and plays an essential role in the establishment and maintenance of the transformed phenotype. Inhibition of IGF1R signaling thus appears to be a promising strategy to interfere with the growth and survival of cancer cells. IGF1R is frequently overexpressed by tumors and mediates proliferation and apoptosis protection. IGF signaling also influences hypoxia signaling, protease secretion, tumor cell motility, and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, IGF1R is now an attractive anti-cancer treatment target.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Bhr C, et al. (2004) The insulin like growth factor-1 receptor (IGF-1R) as a drug target: novel approaches to cancer therapy. Growth Horm IGF Res. 14 (4): 287-95. 2. Riedemann J, et al. (2006) IGF1R signalling and its inhibition. Endocr Relat Cancer. 13 Suppl 1: 33-43. 3. Gualco E, et al. (2009) IGF-IR in neuroprotection and brain tumors. 4. Front Biosci. 14: 352-75.Annenkov A. (2009) The insulin-like growth factor (IGF) receptor type 1 (IGF1R) as an essential component of the signalling network regulating neurogenesis. Mol Neurobiol. 40 (3): 195-215.

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IGF1R/CD221 Protein, Human, Recombinant (His)

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