IGFBP-7 Protein, Human, Recombinant (aa 1-282, hFc)

IGFBP-7 Protein, Human, Recombinant (aa 1-282, hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 53.5 kDa and the accession number is Q16270.

IGFBP-7 Protein, Human, Recombinant (aa 1-282, hFc)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 61,500
100 μg	約5 days	￥ 103,000
200 μg	約5 days	￥ 172,500
500 μg	約5 days	￥ 342,000

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生物学的情報	Immobilized Human CD93 (His Tag) at 2 μg/ml (100 μl/well) can bind Human IGFBP7 (hFc Tag). The EC50 is 8-60 ng/ml.
説明	IGFBP-7 Protein, Human, Recombinant (aa 1-282, hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 53.5 kDa and the accession number is Q16270.

Species	Human
Expression Host	HEK293
Tag	hFc
Accession Number	Q16270
別名	IGFBP-7, IGFBPRP1, IBP-7, TAF, FSTL2, PSF, insulin-like growth factor binding protein 7, MAC25, IGFBP-7v, AGM, RAMSVPS
Construction	A DNA sequence encoding the human IGFBP7 (Q16270) (Met 1-Leu 282) was fused with the Fc region of human IgG1 at the C-terminus.
Protein Purity	≥ 85 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	53.5 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Insulin-like growth factor-binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity, and induce apoptosis in RKO and SW620 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence, and IGF affinity.

参考文献

1. Oh Y,et al.(1996) Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-I and -II. J Biol Chem. 271(48): 30322-5. 2. Wilson EM,et al.(1997) Generation and characterization of an IGFBP-7 antibody: identification of 31kD IGFBP-7 in human biological fluids and Hs578T human breast cancer conditioned media. J Clin Endocrinol Metab. 82(4): 1301-3. 3. Lin J,et al.(2007) Methylation patterns of IGFBP7 in colon cancer cell lines are associated with levels of gene expression. J Pathol. 212(1): 83-90.

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IGFBP-7 Protein, Human, Recombinant (aa 1-282, hFc)

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