IGFBP-7 Protein, Mouse, Recombinant (aa 26-281, His)

IGFBP-7 Protein, Mouse, Recombinant (aa 26-281, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.69 kDa and the accession number is AAH92538.1.

IGFBP-7 Protein, Mouse, Recombinant (aa 26-281, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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説明	IGFBP-7 Protein, Mouse, Recombinant (aa 26-281, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.69 kDa and the accession number is AAH92538.1.

Species	Mouse
Expression Host	HEK293 Cells
Tag	His
Accession Number	AAH92538.1
別名	Mac25, insulin-like growth factor binding protein 7, AGM, Fstl2
Construction	The Human IGFBP7 (AAH92538.1) (Ser26-Leu281) was expressed, with a polyhistidine tag at the N-terminus.
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	27.69 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM MES, 150 mM Nacl, 0.02% Tween 20, 5% Trehalose, 10% Glycerol, pH 5. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Insulin-like growth factor-binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity, and induce apoptosis in RKO and SW620 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence, and IGF affinity.

参考文献

1. Oh Y,et al.(1996) Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-I and -II. J Biol Chem. 271(48): 30322-5. 2. Wilson EM,et al.(1997) Generation and characterization of an IGFBP-7 antibody: identification of 31kD IGFBP-7 in human biological fluids and Hs578T human breast cancer conditioned media. J Clin Endocrinol Metab. 82(4): 1301-3. 3. Lin J,et al.(2007) Methylation patterns of IGFBP7 in colon cancer cell lines are associated with levels of gene expression. J Pathol. 212(1): 83-90.

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IGFBP-7 Protein, Mouse, Recombinant (aa 26-281, His)

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