IgG1 Fc Protein, Human, Recombinant (C103S)

IgG1 Fc Protein, Human, Recombinant (C103S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 26 kDa and the accession number is P01857-1.

IgG1 Fc Protein, Human, Recombinant (C103S)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 38,000

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生物学的情報	1. Measured by its ability to bind human CD16a-His (Cat: TMPY-01964) in a functional ELISA. 2. Measured by its ability to bind human CD16a-AVI-His (Cat: TMPY-01965) in a functional ELISA. 3.Measured by its ability to bind human CD16a-His (Cat: TMPY-00763) in a functional ELISA.
説明	IgG1 Fc Protein, Human, Recombinant (C103S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 26 kDa and the accession number is P01857-1.

Species	Human
Expression Host	HEK293
Tag	Tag Free
Accession Number	P01857-1
別名	Ighg1, IgG1 Fc
Construction	A DNA sequence encoding the human IgG1 Fc region (P01857-1) (Glu99-Lys330) (one aa mutation, 103 Cys/Ser) was expressed.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	26 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

参考文献

1. Carosella ED. et al., 1988, Cell Immunol. 112: 262-70. 2. Eckle I. et al., 1990, Clin Exp Immunol. 81: 352-6. 3. Lorriaine C. et al., 1995, J Biol Chem. 270:8164-71. 4. Gomez-Guerrero C. et al., 2000, J Immunol. 164: 2092-101. 5. Sprague ER. et al., 2004, J Biol Chem. 14: 14184-93. 6. Anthony RM. et al., 2008, Science. 320: 373-6.

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IgG1 Fc Protein, Human, Recombinant (C103S)

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