IgG1 Fc Protein, Mouse, Recombinant (C102S)

IgG1 Fc Protein, Mouse, Recombinant (C102S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25.8 kDa and the accession number is AAK53870.1.

IgG1 Fc Protein, Mouse, Recombinant (C102S)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 59,000
1 mg	約5 days	￥ 271,000

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説明	IgG1 Fc Protein, Mouse, Recombinant (C102S) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25.8 kDa and the accession number is AAK53870.1.

Species	Mouse
Expression Host	HEK293 Cells
Tag	Tag Free
Accession Number	AAK53870.1
別名	Igh-4, IgG1, VH7183
Construction	The Fc region of Mouse IgG1 (AAK53870.1) (Val 98-Lys 324) (one aa mutation, 102 Cys / Ser) was expressed and purified.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	25.8 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

参考文献

1. Carosella ED. et al., 1988, Cell Immunol. 112: 262-70. 2. Eckle I. et al., 1990, Clin Exp Immunol. 81: 352-6. 3. Lorriaine C. et al., 1995, J Biol Chem. 270:8164-71. 4. Gomez-Guerrero C. et al., 2000, J Immunol. 164: 2092-101. 5. Sprague ER. et al., 2004, J Biol Chem. 14: 14184-93. 6. Anthony RM. et al., 2008, Science. 320: 373-6.

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IgG1 Fc Protein, Mouse, Recombinant (C102S)

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